Chemicals and consumables PTE HRNL 5: Molecular biology reagents

2.1. Procedure

Title : Molecular biology reagents

Description : Procurement of molecular biology reagents for the National Laboratory of Human Reproduction of the PTE within the framework of the RRF-2.3.1-21-2022-00012 application as follows: Transferrin Human ELISA Kit, Analytical sensitivity: <1.1 ng/mL, detection range 1.029-750 ng/mL Activin A human ELISA kit, Analytical sensitivity: <16 pg/mL, detection range 20.48-5000 pg/mL Follistatin (FST) Human ELISA Kit, Analytical sensitivity: <501 pg/mL, detection range 0.512-125 ng/mL Caspase 9 Human ELISA Kit, Analytical sensitivity: <0.5 ng/mL, detection range 1.6-100 ng/mL Cytochrome C human ELISA kit, Analytical sensitivity: <0.06 ng/mL, detection range 0.08-5.0 ng/mL human IL-10 ELISA kit, Analytical sensitivity: <1 pg/mL, detection range 7.8-500 pg/mL human TGFb ELISA kit, Analytical sensitivity: <8.7 pg/mL, detection range 31-2,000 pg/mL human IL-4 ELISA kit, Analytical sensitivity: <1.4 pg/mL, detection range 7.8-500 pg/mL Human Active (cleaved) caspase 3 ELISA kit, Analytical sensitivity: <0.034 ng/mL, detection range 0.039-2.5 ng/mL PARP (cleaved) human ELISA kit, Analytical sensitivity: <0.062 ng/mL, detection range 0.156-10 ng/mL Human Granzyme B ELISA kit, Analytical sensitivity: <0.43 pg/mL, detection range 0.7-480 pg/mL Human Fas ligand ELISA kit, Analytical sensitivity: <0.08 ng/mL, detection range 0.16-10.0 ng/mL Antibody: PerCP-eFluor 710 Anti-human CD11c, clone: 3.9 Human MIS/AMH ELISA Kit, Analytical sensitivity: min. 0.18 ng/mL, detection range 0.18-40 ng/mL, intraassay <10% Total Exosome Isolation Reagent (from serum) for column separation Total Exosome RNA & Protein Isolation Kit, technology: Spin Column, sample type: organelle & vesicle samples, exosomes. Human CD63 Antibody, recombinant, monoclonal rabbit IgG, unconjugated, anti-CD63, for direct ELISA. Clone: 2H5I1 Protein G coated plates Transparent, polystyrene, 96 well, ProteinG surface treated, for colorimetric assays. Protein G coated plates Black, polystyrene, 96 well, ProteinG surface treated, for colorimetric assays. Live cell antifade reagent, for live cell imaging, can be transported at room temperature, resistant to at least 4 freeze-thaw cycles. Glass softset antifade mountant A glycerol-based, ready-to-use, fade-retarding mounting solution for glass slides, can be applied directly to fluorescently labeled cell or tissue samples for microscopic analysis. Its refractive index is 1.52 with minimal spherical aberration. It provides sharp, bright images at focal lengths of 0-500 um. MUP (4-Methylumbelliferyl Phosphate, Free Acid), fluorescent substrate for alkaline phosphatase, Excitation/Emission: 360/449 Ready-to-use solution, secondary antibody labeled with alkaline phosphatase enzyme. Anti-rabbit IgG polyclonal antibody conjugated with alkaline phosphatase enzyme label. Reactivity: rabbit. Ready-to-use solution,secondary antibody labeled with alkaline phosphatase enzyme. Anti-mouse IgG polyclonal antibody conjugated with alkaline phosphatase enzyme marker. Reactivity: mouse. AP chemiluminescent substrate (CDPStar). Non-radioactive, ready-touse CDPStar solution for chemiluminescent immunodetection using alkaline phosphatase marker, western blot or dot blot. Optical adhesive film compatible with Thermo Fisher Fast-advanced 96 well PCR plate, one package contains 100 films. Cell freezing medium, suitable for freezing mammalian cells cultured in at least DMEM, DMEM/F-12, MEM, RPMI 1640, and Ham's F-12 medium. With standard serum content, 10% DMSO, high glucose content, and phenol red, sterile. Finnpipette compatible filter pipette tip, 5 ml volume Trypsin/Lys-C Mix 5X20ug 96-well, transparent polystyrene, plate, maxisorp surfacetreated plate, sterile, not certified, Working volume: 350 ul Calibration solution ESI positive Calibration solution for positive polarity ESI ionization Ready to use, contains: caffeine, Met-Arg-Phe-Ala amino acids and, ultramark 1621. Calibration solution ESI negative Calibration solution for negative polarity ESI ionization Ready to use, contains: SDS and sodium taurocholate, and ultramark 1621. Abundant protein depletion columns Disposable centrifuge column suitable for depletion of the 14 highestconcentrated proteins in biological samples. In the case of albumin and IgG fractions, it has a depletion efficiency of over 95%. Within the framework of the offer, the Bidder may submit an offer for products with technical parameters equivalent to or better than those contained in the technical description. The contracting authority draws attention to Section 46 (3) of Government Decree 321/2015. (X.30.). References to specific manufactures and types in the call for tenders and in the public procurement documents are made only for the purpose of clearly defining the subject of the public procurement. If the call for tenders and the public procurement document contain a reference to a specific manufacture, origin, type of thing or activity, the contracting authority shall accept a product that is equivalent in all respects pursuant to Section 46 (3) of Government Decree 321/2015. (X.30.). 

Internal identifier: EKR000481212024/5

Identifier of the contract: 5

5.1.3. Estimated duration
Duration: 60 Days