MiDA - Mitochondrial Dysfunction Assays: In vitro assay platform for the identification of new pharmacological compounds active on mitochondrial dysfunctions related to neurodegenerative and oncological diseases

Description: The MiDA project has allowed Axxam to develop an automated screening platform for the integrated study of mitochondrial functions. The platform can be used to analyze the mitochondrial dysfunctions involved in various human disease such as aging, neurological diseases (Alzheimer's, Parkinson's, multiple sclerosis, ALS), cancer and metabolic disorders, and it is suitable for the identification of novel therapeutic compounds. Several assays have been developed to measure mitochondrial metabolism (ATP production and ROS generation), function (mitochondrial membrane potential) and the health and quality of mitochondria (mitophagy reporter assay). Finally, a state-of-art protocol has been developed to isolate mitochondria and to characterize the electrophysiological properties at the single channel resolution. The established assays have been validated by testing a commercial library of compounds involved in mitochondrial pathways.